Blood collection in pediatrics ppt

Blood collection and preservation - SlideShar

Blood collection and preservation 1. COLLECTION &PRESERVATION OF BLOOD 2. It is a blood collection used an all labs to find out why symptoms are appear in the patient Phlebotomy(to cut a vein in Greek) is the process of making incision in a vein. A person who practices phlebotomy is termed as phlebotomist • Always mix by gentle inversion after collection. • A discard tube MUST be used if specimen is to be drawn using a winged blood collection set. This is to remove air from the collection devise to ensure proper blood volume is obtained in the tube. • Specimens MUST be filled to the line for adequate equilibration. DO NOT overfill Vacuum Blood Collection . Terry Kotrla, MS, MT(ASCP)BB; 2 Introduction. The vacuum blood collection system consists of a double-pointed needle, a plastic holder or adapter, and a series of vacuum tubes with rubber stoppers of various colors. The evacuated tube collection system will produce the best blood samples for analysis. The blood goes.

blood the day of surgery. They were rude and had NO compassion. zBlood had to be taken twice for the same test b/c not enough blood was taken the first time - zI was VERY upset with the nurse's responses when I warned them that our daughter was a hard stick - it took over 30 min. to get an IV line in after they tried feet, hands, etc Introduction To Blood Collection Tubes - The blood collection vacuum tubes have been designed for a certain amount of blood to be drawn into the tube by vacuum according to the amount of pre-filled anticoagulant in the tube. The PowerPoint PPT presentation: Specimen collection is the property of its rightful owner Collection of blood from Neonates ages 0 through 3 months, this must be done by an experienced phlebotomist or a pediatric nurse. Heel stick is the only acceptable manner of collection for laboratory personnel in this age group unless infant weight is 21 pounds or greater than an arm draw can be performed Blood tests can yield valuable information about the patient's nutrition, hematologic, metabolic, immune, and biochemical status. Blood culture specimens are obtained by venipuncture, which involves inserting PEDIATRIC PPT LECTURES. General Pediatrics Collection. ADHD (Coll) Allergic Rhinitis (Waibel) Croup (Dobson) Head Lice (Gorman) Pediatric Environmental Health (Johnson) Pediatric Jeopardy Template (Gorman

Appropriate Maximum Phlebotomy Volumes Purpose: To provide a guideline for performing safe phlebotomy procedures on our neonate, infant and pediatric population. Scope: All personnel drawing blood specimens, i.e., by direct phlebotomy or venous/arterial lines. Procedure: The following chart outlines the guidelines for blood drawing that is practiced a COLLECTION OF BLOOD SPECIMENS MANUAL Page 2 of 27 1. INTRODUCTIO: 1.1. Scope: To standardize the venous and arterial blood specimen collection procedures for medical laboratory testing. These guidelines are appropriate for adoption by all health care providers responsible for blood specimen's collection in outpatient and inpatient setting. 1.2 Pediatric 20 ml 2.5 - 10 ml 2.5 - 10 ml Adult 20 ml 10 ml 10 ml Syringe Aerobic bottle Anaerobic bottle needed Do not overfill bottles (do not add more than 10 ml of blood to each bottle) *In some cases, it may not be possible to obtain 20 ml blood from an adult. If 10 ml or less is obtained, place all of the blood in the aerobic bottle Pediatric Weight Chart for Blood Collection - 12.2016 WEIGHT Maximum mL amount to be todrawn in 24 hours mL amount be drawn in 30 days Kg lbs. (Kilograms) (pounds) 2.5% of total blood vol ume5% 1 2.2 2.5 6 2 4.4 5 10 3 6.6 6 12 4 8.8 8 15 5 1011 20 6 13.2 12 24 7 15.4 14 28. Instructions for Blood Culture Collection Revised 7/2019 Page 1 of 2 Step 1 - Collection Preparation Obtain BLOOD CULTURE COLLECTION KITS - (one kit for each venipuncture) - Central Supply stocks as follows: Lawson #127919 collection kit. Individual bottles are stocked as anaerobic bottle Lawson #70, and Aerobic bottle Lawson #71

Phlebotomy Tubes Explained - E Phlebotomy TrainingFree Blood Red Medical PowerPoint Template for Medical

The blood culture collection bottle will probably indicate a desired volume of sample for optimal results, but 1 mL of blood is generally acceptable for a small infant. Wash off the cleanser promptly after blood has been collected because iodine solutions and other detergents can irritate infant skin 1. Bulent Ozgonenel, MD* 2. Tammon A. Nash, MD, MS* 3. Madhvi Rajpurkar, MD* <!-- --> 1. *Wayne State University School of Medicine, Children's Hospital of Michigan, Detroit, MI Blood transfusion plays an important role in the treatment of sick children, just as do pharmaceutical medications, intravenous fluids, and nutritional supplements. Unlike these therapeutic tools, however, blood is. SPECIMEN COLLECTION: SERUM, PLASMA, OR BLOOD. Draw blood in the color-coded vacutainer tube indicated in the alphabetical test listing. For serum or plasma, draw approximately 2 1/2 times the requested volume. For serum, allow the blood to clot at least ten minutes and separate by centrifugation Mitra ® devices with VAMS ® technology are used for remote blood collection to monitor therapeutic drugs and malnutrition. Garner the logistical benefits of using Mitra ® devices vs. DBS cards in low-resource regions around the globe. Simplify blood collection in pediatric study subjects by switching to at-home finger-prick sampling to.

PPT - Vacuum Blood Collection PowerPoint presentation

  1. Preparation is Key for Pediatric Blood Draws. Each day, phlebotomists are tasked with putting patients at ease during a blood draw. This can be especially challenging when it comes to pediatric patients. Sue McAnany, MT, phlebotomy education coordinator at URMC, says her trainees are instructed to give special care to younger patients and.
  2. History 1972 USA - first attempt to transplant 1. Ende M, Ende N. Hematopoietic transplantation by means of fetal (cord) blood: A new method. Va Med Mon, 1972; 99: 276-280. 1988 Paris - first UCB transplantation zGluckman E, Broxmeyer HA, Auerbach AD, Friedman HS, Douglas GW, Devergie A, Esperou H, Thierry D, Socie G, Lehn P, et al. Hematopoieti
  3. Appointments are required for blood collection service. This location offers online appointment bookings. Visit https://booking.nshealth.ca to book your own appointment.. Patients can book their own appointment for blood collection service by calling 902-928-2830 from 8:30 a.m. to 2:30 p.m. Monday to Friday (excluding holidays)
  4. The purpose of the Pediatric Bone Marrow Stem Cell Manual is to present, in a clear and unrelated umbilical cord blood Syngeneic identical twin Autologous patient Technique of Bone Marrow Collection The bone marrow donor is taken to the operating room, where under general anesthesia,.
  5. This tube contains ACD, which is used for the collection of whole blood for special tests. NOTE: After the tube has been filled with blood, immediately invert the tube 8-10 times to mix and ensure adequate anticoagulation of the specimen. Pearl white-top tube plasma preparation tube (PPT
  6. The BD Vacutainer® Plasma Preparation Tube (PPT™) is a plastic evacuated tube used for the collection of venous blood in order to prepare undiluted plasma for use in molecular diagnostic testing. The BD PPT™ should be at room temperature and properly labeled for patient identification
  7. g from a lack of knowledge of the bacterial load and because of.
PPT - Screening for Transfusion transmissible Infections

PPT - Specimen collection PowerPoint presentation free

blood volume in intravascular volume depletion from acute blood loss or systemic disease. A rarer indication is to suppress endogenous hemoglobin and red blood cell production in selected patients with thalassemia or sickle cell disease (SCD). Table 1 (page 19) and Table 2 (page 20) present some guidelines for transfusion of pediatric patients Blood collection systems for pediatrics. 2 3 Our smallest patients The demands placed on blood collection systems in this particular group are quite unique. Considering WKHVH UHTXLUHPHQWV ZH GHYHORSHG SHGLDWULF VSHFL 1F FROOHFWLRQ V\VWHPV WKDW PDNH WKH SURFHVV RI FROOHFWLQ xxx00.#####.ppt 9/17/2019. P. Pediatrics The road to curing most children with acute lymphocytic leukemia (ALL), the most common childhood cancer, may be the greatest success story in the history of cancer. -America Society of Hematology (Dec 2008) CORE BIOPSY, CLOT AND ASPIRATE, AND PERIPHERAL BLOOD SMEAR: RELAPSED B-ACUTE. Describe how to process blood specimens for testing TERMINOLOGY Anticoagulant:substance that prevents clotting (EDTA and Heparin) Hematocrit: the % of RBC in blood Hemoglobin: iron containing protein of blood that carries oxygen Hemolysis: breakdown of blood cells Lumen: the diameter of. e.g. needle or vein Phlebotomy: blood collection using.

The use of low-volume collection tubes is also recommended to minimize the vacuum pressure that could otherwise cause vein collapse and minimize the volume of blood needed. For detailed information about performing pediatric phlebotomy procedures consult CLSI's GP41— Collection of Diagnostic Venous Blood Specimens PPT™ is an evacuated, sterile blood collection tube that contains an inert gel and spray-dried K2EDTA anticoagulant for achieving plasma separation. When the sample-filled tube is subjected to centrifugation, the gel migrates and forms a physical barrier between the plasma and most of the cellular elements. Since the tube uses spray-dried K2EDTA as opposed to liquid additives, the plasma. Special considerations for collections from vascular access devices, blood culture collection, and collections in isolation environments are included. References. 1 Walco GA. Needle pain in children: contextual factors. Pediatrics. 2008:122(Suppl 3):S125-S129. 2 Cohen LL. Behavioral approaches to anxiety and pain management for pediatric venous. It is recommended that: Red cells are transfused at up to 5 mL/kg/h (unless there is active major bleeding) and the transfusion should be completed within 4 hours (see Chapter 4). Apheresis platelets should be used for all children <16 years old to reduce donor exposure. The typical dose for children weighing less than 15 kg is 10-20 mL/kg

Reducing the Blood Culture Contamination Rate in a

o Adhesive bandages / tape - protects the venipuncture site after collection. o Needle disposal unit - needles should NEVER be broken, bent, or recapped. Needles should be placed in a proper disposal unit IMMEDIATELY after their use. o Gloves - can be made of latex, rubber, vinyl, etc.; worn to protect the patient and the phlebotomist. o Syringes - may be used in place of the evacuated. Blood collection in scared child teacher tayo kaye humanized collectionblood collection with syringeRecolección de sangre en niño asustado maestro tayo kaye.

Blood Transfusion Reaction ppt. Blood Donors-collection and storage -1 Blood Donors-collection and storage -2 Click here for other medic... OBSTETRICS AND GYNAECOLOGY (OBG) - POWERPOINT PRESENTATIONS Pain treatment ppt. Pediatric Anesthesia ppt. Pediatric Patients ppt. PEDICULOSIS ppt. PHARYNGITIS ppt. POST MORTEM CHANGES ppt. Proteinuria. I. OBTAINING BLOOD SPECIMENS FROM INFANTS 1. Warm the patient's foot with a warming device or towel (do not exceed 42 degrees C) for 10 minutes before obtaining sample. 2. Assemble the appropriate tubes for blood collection. If a pediatric blood collection containe Blood culture series: a group of temporally related blood cultures that are collected to determine whether a patient has bacteremia or fungemia. Blood culture set: the combination of blood culture bottles (one aerobic and one anaerobic) into which a single blood collection is inoculated

This chapter covers all the steps recommended for safe phlebotomy and reiterates the accepted principles for blood drawing and blood collection ().The chapter includes background information (Section 2.1), practical guidance (Section 2.2) and illustrations (Section 2.3) relevant to best practices in phlebotomy.The information given in this section underpins that given in the remainder of Part. In this video presentation, I will be discussing PKU, Heel Sticks, Blood Volume collection for infants/neonates and Blood Culture Procedures. Preparing phle.. Allogeneic Donor Selection And Blood Collection PPT. Presentation Summary : The donor screening process is one of the most important steps in protecting the safety of the blood supply. It is, therefore, imperative that proper guideline Four Types Of Blood Specimens. 1. Intravenous whole blood: The most widely used whole blood blood sample comes from the vein. The site of blood collection is the anterior cubital vein and the wrist vein. In children and neonates, the jugular vein and the femoral vein are sometimes used. 2

Pediatric Hip: Anatomy • Lateral Circumflex - Supplies the anterior portion of the femoral epiphysis and physis until 5-6 months of age - Contribution to femoral head blood supply diminishes by 3 years of age • Medial Circumflex - Major blood supply to proximal femur - The entire blood supply to the proxima Note that if a winged blood collection set (butterfly) is used and the coagulation tube is the first tube drawn, a discard blue top tube should be used to clear the dead-space volume of the line or the line should be allowed to 'fill' prior to attaching the vacutainer adapter. This will ensure that an adequate blood volume is collected Policies and Procedures: Central Venous Catheters - PICC, Short Term, Tunneled, I.D. #1042 Implanted - Blood Withdrawal Page 2 of 10 Peripherally Inserted Central Catheter (PICC): A central venous access device inserted into a peripheral vein whose tip dwells in the superior vena cava and is used in acute care, long term care or home care This blog contains a compilation of lecture notes of various medical subjects. Most of them are in .ppt format and .pdf format. This blog will be helpful for Medical, Dental and Paramedical students in understanding various topics which are prepared by the topmost Doctors in the medical field Appropriate blood volumes for collection. 5-10% of body weight = total blood volume. The circulating blood volume can generally be estimated as 55-70 ml/kg of total body weight. However, care should be taken in these calculations as the percent of total blood volume will be lower (-15%) in sick, obese, and older animals

Kaditis AG, O'Marcaigh AS, Rhodes KH, et al. Yield of positive blood cultures in pediatric oncology patients by a new method of blood culture collection. Pediatr Infect Dis J 1996; 15:615. Miller JM, Binnicker MJ, Campbell S, et al Smaller collection tubes for sample sizes of 2 mL or less may be appropriate in situations where a smaller amount blood should be drawn, as in pediatric patients, or to minimize hemolysis during collection, or to avoid insufficient sample volume in the collection tube Materials needed for Blood Collection: 1. Gloves 2. 70% isopropyl alcohol pads 3. Dry sterile gauze pads 4. Sterile sticking device with a point not greater than 2.0 mm in depth (the most effective method is the use of scalpel bladed lancets) 5. Newborn Screening filter paper collection form (CL-89) with protective envelope Bleeding Procedure: 1

Red Blood Cell Transfusion Strategies in Adult and Pediatric Patients with Malignancy. Hematol Oncol Clin North Am 2016; 30:529. Lightdale JR, Randolph AG, Tran CM, et al. Impact of a conservative red blood cell transfusion strategy in children undergoing hematopoietic stem cell transplantation indian pediatrics 489 volume 55__june 15, 2018 umbilical cord blood banking: consensus statement of the indian academy of pediatrics anupam sachdeva1, v inod gunasekaran1, payal malhotra2, dinesh bhurani2, s atya prakash yadav3, nita radhakrishnan4, manas kalra5, s unil bhat6, r uchira misra3 and pramod jog7; for the 'guidelines on umbilical cord blood banking' committee of indian a cademy. a. Safety Lock Vacutainer Blood Collection Set with 12-inch tubing and multisample luer adapter. The most commonly used is the 21g or 23g 3/4 inch set. A 25g ¾ inch set is available for pediatric and special needs patients. b. Disposable vacutainer holder or blood culture collection BactiAlert adapter cap and adapter insert. 2 Hyperglycemia generally is defined as a whole blood glucose concentration greater than 6.66 to 6.94 mmol/L (>120 to 125 mg/dL) or a plasma glucose concentration greater than 8.05 to 8.33 mmol/L (>145 to 150 mg/dL), regardless of the neonate's gestational age, weight, or postnatal age

Collection and Inoculation of Blood Specimens for Routine Culture (Bacterial, T.B. or Fungus) Policy Whenever possible, a peripheral sample is preferred over a sample obtained from a line, unless a line infection is suspected.When drawing multiple blood samples from a line, always draw the blood culture first Venous blood gas. This can be used in some diseases (neonatal sepsis/respiratory distress syndrome [RDS]) to diagnose acid-base imbalance if an arterial blood gas can't be obtained. Although arterial blood gas is preferred, venous samples show good validity in terms of pH, Pco 2, and HCO 3. + +

Blood Collection: Babies and Childre

Blood Specimen Collection and Processing. The first step in acquiring a quality lab test result for any patient is the specimen collection procedure. The venipuncture procedure is complex, requiring both knowledge and skill to perform. Several essential steps are required for every successful collection procedure Blood Collection methods in Mice and Rats (anesthetized): NB: The following methods require anesthesia (local and/or general depending on species and technique) to relieve pain and distress associated with the technique or for restraint.Use of these methods may require scientific justification for why less painful techniques cannot be used in the animal care and use protocol Blood Culture Volume Guidelines with References, Abstracted from Phlebotomy Procedures PHL-6 and PHL-12 . For general Nursing guidelines see collection instructions and protocol N-03.020 and relevant pediatric guidelines. Pediatric Patients: Microbiology requires a minimum of 1mL per year of age. Phlebotomists are welcome to use the alternate char Background Pediatric intravenous (IV) cannulation is an integral part of modern medicine and is practiced in virtually every health care setting. Venous access allows the sampling of blood, as well as administration of fluids, medications, parenteral nutrition, chemotherapy, and blood products Blood Specimen CollectionDefinitionBlood specimen collection is performed routinely to obtain blood for laboratory testing. Blood can be obtained from venous access devices and sometimes by fingerstick. Blood is most frequently obtained via a peripheral vein puncture (venipuncture). Source for information on Blood Specimen Collection: Gale Encyclopedia of Nursing and Allied Health dictionary

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If 15% to 20% of the blood volume is removed, cardiac output and blood pressure will be reduced. Removal of 30% to 40% can induce shock and death. It is extremely important to apply pressure to the blood collection site, especially when penetrating an artery, for at least 3 to 5 minutes post blood collection to prevent hematoma formation supplementation with fresher whole blood units or blood components, especially platelets, may be necessary. Fresh whole blood (FW) refers to W collected on an emergency basis from a walking blood bank (W). FWB can either be stored at room temperature and used within 24 hours of collection (and then destroyed if no

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Pediatric Vascular Access and Blood Sampling Techniques

Pediatric ECMO is a treatment system that functions in the same way as a regular ECMO does. The system uses a pump to circulate blood out of the body to oxygenate the blood, remove carbon dioxide, and pump it back into the blood vessels of a very sick child or baby Vacutainer® BD™ The BD™ Vacutainer® venous collection consists of the Push Button Blood Collection Set designed to help protect you and your practice from needlestick injuries. The Vacutainer® Blood Collection Tubes, both plastic and glass, are engineered to reduce specimen spillage.The Safety-Lok™ Blood Collection Sets and the Eclipse™ Blood Collection Needles feature a patented. RESULTS. The total number of samples was 578, with 309 obtained from infants located in the NICU and 269 samples from IICU infants. The mean volume of blood overdraw for all blood tests was 19.0% ± 1.8% (P < .0001).The relative volume of blood drawn differed markedly based on individual laboratory tests ().For 4 of the 11 blood tests, significantly (P < .05) more blood was drawn than was. 1. Gather the necessary collection blood bottles or tubes. 2. Swab the tops of each blood culture bottle and/or the stopper of an SPS tube with alcohol. Do not allow alcohol to pool, as it could enter the system and kill organisms. Allow to dry while preparing the patient. 3. Cleanse the skin with 70-95% ALC. 4. Cleanse the skin with 1-2% TOI

Low sample volume: Collection of a small blood volume (e.g. 0.5-1 mL) with placement into a standard 5 mL EDTA tube will cause shrinkage of red blood cells, because EDTA is hypertonic. This will cause a false decrease in the mean cell volume (MCV) and false increase in mean cell hemoglobin concentration (MCHC) of red blood cells The blood bags cover a wide range of blood bag configurations, from single blood bags to blood bags with filters for leucoreduction. The blood bags meet blood transfer needs with sizes ranging from pediatric to 1000 mL, and help to improve lab efficiency by simplifying stock management and ordering with a long product shelf-life The maximum permitted blood volume includes blood lost during collection. As a general rule, 20 drops = 1 mL (i.e. 5 drops = 250 uL) Maximal blood collection limits are as follows: No more than 1% of the animal's body weight in one collection or over a 24 hour period. For example: 25g mouse x 1% = 0.25mL or 250uL maximum blood remova

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Start studying Blood Collection Equipment, Additives, and Order of Draw by Sir George. physically separates the liquid component of the blood from the cells (densities of 1.03 and 1.09 g/cm3respectively) after centrifugation, and forms a barrier between the liquid phase and the cells. Capillary specimens collection. is especially useful in. CBC/blood smear results with any cells seen that are less mature than a myelocyte are almost always associated with hematological malignancy. This warrants immediate hematology/oncology Microsoft PowerPoint - Sovie Center pediatric GR Lab Value Presentation.pptx [Read-Only

Specimen-handling : Pediatri

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Advancements in Pediatric Blood Sampling The VAMS

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BabyLady, BSN, RN. Specializes in NICU, Post-partum. Apr 9, 2010. I float down to Peds sometimes and I don't have very good luck with toe sticks, mainly because I can't get the flow of blood enough and heel sticks are much better so you don't get air in your CBG draw. There are also more nerve endings in toes than heels, so it's a pain factor. Neonatal/Pediatric Deltran® Plus is a closed blood collection system that is designed specifically to conserve blood and protect the neonatal patient from infection. The standards for accuracy, patient safety and ease of use you get with Deltran® disposable blood pressure transducers and Deltran® Plus arterial blood collection systems are. Pediatric venous thromboembolism (VTE) is an ever-increasing phenomenon leading to significant complications and death in children and touching every aspect of pediatric medicine. 1 There are several notable differences between VTE in children and adults. First, the majority of thrombotic events in children are caused by central venous catheters (CVC), and as a corollary to this fact, there is. 2.2 Blood safety in the hospital setting 9 2.3 Blood donor recruitment 9 2.4 Blood collection 10 3 Blood components 11 3.1 Whole blood 11 3.2 Red cell concentrates / packed red blood cells 12 3.3 Platelet concentrates 12 3.4 Fresh frozen plasma 1

PPT - Pediatric Acute Kidney Injury: Supportive Therapies

NOTE: When using a winged blood collection set for venipuncture and a coagulation tube is the first tube needed, first draw a discard tube (plain red top or light blue top). The discard tube does not need to be filled completely. 1. Assemble the necessary equipment appropriate to the patient's physical characteristics EAUrf SOP Collection of Blood V1 26Jun2018 SOP COLLECTION OF BLOOD The following blood specimens are collected using vacutainers: Sample no. Specimen Blood volume (ml) Becton Dickinson Tubes B01 Serum 8.5 Vacutainer SST II Advance B02 Serum 8.5 Vacutainer SST II Advance B03 Plasma (EDTA) 8.5 Vacutainer PPT K 2 EDTA 15.8 m A Google search was performed of guidelines for blood collection volume for pediatric research in Canada. Results: No results were returned from the OVID/pubmed search. No guidance from Health Canada was found regarding the safe volume of blood collection from pediatric patients in research. No evidence-based Canadian studies were located 1. Physicians should wear gowns, masks, and gloves during specimen collection. 2. Prepare skin as for Blood Culture. 3. Drape the surrounding skin with sterile linen. 4. Aspirate the marrow percutaneously using a sterile needle and syringe. 5. Transfer 3-5 ml for each: Bacterial culture requests, inoculate into a blood culture bottle - do not sen Remove and discard your gloves and wash your hands thoroughly. Allow the patient to rinse out his mouth and provide a tissue. Record the amount, consistency, and color of the sputum collected, as well as the time and date in the nursing notes. Send the sample to the lab immediately, without refrigeration. 3 Blood collection tubes should be drawn in a specific order to avoid cross-contamination of additives . An important early decision in blood collection is whether to collect anticoagulated blood (consisting of plasma, buffy coat, and RBCs) or coagulated blood (consisting of serum and RBC clot; refs. 1, 5, 6). There are several types of.